autophagy inhibitor ca 5f (MedChemExpress)
Structured Review

Autophagy Inhibitor Ca 5f, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/autophagy+inhibitor+ca+5f/CA-5f/pmc13042881-55-8-16
Average 94 stars, based on 10 article reviews
Images
1) Product Images from "Apigenin Protects H9c2 Cells Against Oxygen–Glucose Deprivation/Reperfusion Injury by Regulating Autophagy via the HIF ‐1α/miR‐20a Axis"
Article Title: Apigenin Protects H9c2 Cells Against Oxygen–Glucose Deprivation/Reperfusion Injury by Regulating Autophagy via the HIF ‐1α/miR‐20a Axis
Journal: Pharmacology Research & Perspectives
doi: 10.1002/prp2.70224
Figure Legend Snippet: Effects of CA‐5f on OGD/R‐induced viability, SOD activity, LDH, MDA, GSH levels, and autophagy marker protein expression in H9c2 cells. (A) The viability of H9c2 cells treated with CA‐5f (10 μM) was assessed by MTT assay. (B, C) LDH leakage, SOD activity levels in H9c2 cells. (D) Cellular ROS levels after OGD/R and CA‐5f (10 μM) application (scale bar: 100 μm). (E, F) Levels of MDA and GSH in different treatment groups. (G) The expression of autophagy‐related proteins (LC3II/I, p62) was detected by Western blot. ( n = 5). * p < 0.05.
Techniques Used: Activity Assay, Marker, Expressing, MTT Assay, Western Blot
Related Articles
MTT Assay:Article Title: Apigenin attenuates myocardial ischemia-reperfusion injury through miR-448/SIRT1 axis Article Snippet: .. Cobalt chloride (CoCl 2 ) (Aladdin Biochemical Technology Co., Ltd, Shanghai, China), DMEM high-sugar medium and DMEM sugar-free medium (Gibco Company, USA), FBS (South American, LONSERA company), API (Yuanye Biotechnology Co., Ltd, Shanghai, China), 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) powder (Solaibao Biotechnology Co., Ltd, Beijing, China), caspase-3 enzyme-linked immunosorbent assay (ELISA) kit (ET Life Sciences R&D Co., Ltd), superoxide dismutase (SOD) and lactate dehydrogenase (LDH) measure kit (Jiancheng Bioengineering Research Institute, Nanjing, China), Trizol reagent, ABScript II cDNA Strand Synthesis Kit, Universal SYBR Green Fast qPCR Mix (Aibotech Biotechnology Co., Ltd, Wuhan, China), SIRT1 primers (Dingguo Changsheng Biotechnology Co., Ltd, Beijing, China), miRNA reverse transcription and qPCR kits, miR-448 primers (Hefei, Zhien Biotechnology Co., Ltd), BCA protein quantitation assay kit (Biyuntian Biotechnology Co., Ltd), Primary antibodies: hypoxia inducible factor 1α (HIF-1α) (USA, Affinity Bioscience), SIRT1, Sequestosome 1 (SQSTM1/p62), LC3, Bcl-2, Bcl-2-associated X protein (Bax), caspase-3, β-actin (Aibotech Biotechnology Co., Ltd, Wuhan, China), Secondary antibodies: horseradish peroxidase (HRP) linked anti-rabbit IgG and anti-mouse IgG (Soleibao Biotechnology Company, Beijing, China), Lipofectamin TM 2000 (Invitrogen Company, USA), OPTI MEM medium (Gibco Company, USA), miR-448 mimic/inhibitor or NC (Jima Pharmaceutical Technology Co., Ltd, Shanghai, China), SIRT1 inhibitor EX-527 and Enzyme-linked Immunosorbent Assay:Article Title: Apigenin attenuates myocardial ischemia-reperfusion injury through miR-448/SIRT1 axis Article Snippet: .. Cobalt chloride (CoCl 2 ) (Aladdin Biochemical Technology Co., Ltd, Shanghai, China), DMEM high-sugar medium and DMEM sugar-free medium (Gibco Company, USA), FBS (South American, LONSERA company), API (Yuanye Biotechnology Co., Ltd, Shanghai, China), 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) powder (Solaibao Biotechnology Co., Ltd, Beijing, China), caspase-3 enzyme-linked immunosorbent assay (ELISA) kit (ET Life Sciences R&D Co., Ltd), superoxide dismutase (SOD) and lactate dehydrogenase (LDH) measure kit (Jiancheng Bioengineering Research Institute, Nanjing, China), Trizol reagent, ABScript II cDNA Strand Synthesis Kit, Universal SYBR Green Fast qPCR Mix (Aibotech Biotechnology Co., Ltd, Wuhan, China), SIRT1 primers (Dingguo Changsheng Biotechnology Co., Ltd, Beijing, China), miRNA reverse transcription and qPCR kits, miR-448 primers (Hefei, Zhien Biotechnology Co., Ltd), BCA protein quantitation assay kit (Biyuntian Biotechnology Co., Ltd), Primary antibodies: hypoxia inducible factor 1α (HIF-1α) (USA, Affinity Bioscience), SIRT1, Sequestosome 1 (SQSTM1/p62), LC3, Bcl-2, Bcl-2-associated X protein (Bax), caspase-3, β-actin (Aibotech Biotechnology Co., Ltd, Wuhan, China), Secondary antibodies: horseradish peroxidase (HRP) linked anti-rabbit IgG and anti-mouse IgG (Soleibao Biotechnology Company, Beijing, China), Lipofectamin TM 2000 (Invitrogen Company, USA), OPTI MEM medium (Gibco Company, USA), miR-448 mimic/inhibitor or NC (Jima Pharmaceutical Technology Co., Ltd, Shanghai, China), SIRT1 inhibitor EX-527 and SYBR Green Assay:Article Title: Apigenin attenuates myocardial ischemia-reperfusion injury through miR-448/SIRT1 axis Article Snippet: .. Cobalt chloride (CoCl 2 ) (Aladdin Biochemical Technology Co., Ltd, Shanghai, China), DMEM high-sugar medium and DMEM sugar-free medium (Gibco Company, USA), FBS (South American, LONSERA company), API (Yuanye Biotechnology Co., Ltd, Shanghai, China), 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) powder (Solaibao Biotechnology Co., Ltd, Beijing, China), caspase-3 enzyme-linked immunosorbent assay (ELISA) kit (ET Life Sciences R&D Co., Ltd), superoxide dismutase (SOD) and lactate dehydrogenase (LDH) measure kit (Jiancheng Bioengineering Research Institute, Nanjing, China), Trizol reagent, ABScript II cDNA Strand Synthesis Kit, Universal SYBR Green Fast qPCR Mix (Aibotech Biotechnology Co., Ltd, Wuhan, China), SIRT1 primers (Dingguo Changsheng Biotechnology Co., Ltd, Beijing, China), miRNA reverse transcription and qPCR kits, miR-448 primers (Hefei, Zhien Biotechnology Co., Ltd), BCA protein quantitation assay kit (Biyuntian Biotechnology Co., Ltd), Primary antibodies: hypoxia inducible factor 1α (HIF-1α) (USA, Affinity Bioscience), SIRT1, Sequestosome 1 (SQSTM1/p62), LC3, Bcl-2, Bcl-2-associated X protein (Bax), caspase-3, β-actin (Aibotech Biotechnology Co., Ltd, Wuhan, China), Secondary antibodies: horseradish peroxidase (HRP) linked anti-rabbit IgG and anti-mouse IgG (Soleibao Biotechnology Company, Beijing, China), Lipofectamin TM 2000 (Invitrogen Company, USA), OPTI MEM medium (Gibco Company, USA), miR-448 mimic/inhibitor or NC (Jima Pharmaceutical Technology Co., Ltd, Shanghai, China), SIRT1 inhibitor EX-527 and Real-time Polymerase Chain Reaction:Article Title: Apigenin attenuates myocardial ischemia-reperfusion injury through miR-448/SIRT1 axis Article Snippet: .. Cobalt chloride (CoCl 2 ) (Aladdin Biochemical Technology Co., Ltd, Shanghai, China), DMEM high-sugar medium and DMEM sugar-free medium (Gibco Company, USA), FBS (South American, LONSERA company), API (Yuanye Biotechnology Co., Ltd, Shanghai, China), 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) powder (Solaibao Biotechnology Co., Ltd, Beijing, China), caspase-3 enzyme-linked immunosorbent assay (ELISA) kit (ET Life Sciences R&D Co., Ltd), superoxide dismutase (SOD) and lactate dehydrogenase (LDH) measure kit (Jiancheng Bioengineering Research Institute, Nanjing, China), Trizol reagent, ABScript II cDNA Strand Synthesis Kit, Universal SYBR Green Fast qPCR Mix (Aibotech Biotechnology Co., Ltd, Wuhan, China), SIRT1 primers (Dingguo Changsheng Biotechnology Co., Ltd, Beijing, China), miRNA reverse transcription and qPCR kits, miR-448 primers (Hefei, Zhien Biotechnology Co., Ltd), BCA protein quantitation assay kit (Biyuntian Biotechnology Co., Ltd), Primary antibodies: hypoxia inducible factor 1α (HIF-1α) (USA, Affinity Bioscience), SIRT1, Sequestosome 1 (SQSTM1/p62), LC3, Bcl-2, Bcl-2-associated X protein (Bax), caspase-3, β-actin (Aibotech Biotechnology Co., Ltd, Wuhan, China), Secondary antibodies: horseradish peroxidase (HRP) linked anti-rabbit IgG and anti-mouse IgG (Soleibao Biotechnology Company, Beijing, China), Lipofectamin TM 2000 (Invitrogen Company, USA), OPTI MEM medium (Gibco Company, USA), miR-448 mimic/inhibitor or NC (Jima Pharmaceutical Technology Co., Ltd, Shanghai, China), SIRT1 inhibitor EX-527 and Reverse Transcription:Article Title: Apigenin attenuates myocardial ischemia-reperfusion injury through miR-448/SIRT1 axis Article Snippet: .. Cobalt chloride (CoCl 2 ) (Aladdin Biochemical Technology Co., Ltd, Shanghai, China), DMEM high-sugar medium and DMEM sugar-free medium (Gibco Company, USA), FBS (South American, LONSERA company), API (Yuanye Biotechnology Co., Ltd, Shanghai, China), 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) powder (Solaibao Biotechnology Co., Ltd, Beijing, China), caspase-3 enzyme-linked immunosorbent assay (ELISA) kit (ET Life Sciences R&D Co., Ltd), superoxide dismutase (SOD) and lactate dehydrogenase (LDH) measure kit (Jiancheng Bioengineering Research Institute, Nanjing, China), Trizol reagent, ABScript II cDNA Strand Synthesis Kit, Universal SYBR Green Fast qPCR Mix (Aibotech Biotechnology Co., Ltd, Wuhan, China), SIRT1 primers (Dingguo Changsheng Biotechnology Co., Ltd, Beijing, China), miRNA reverse transcription and qPCR kits, miR-448 primers (Hefei, Zhien Biotechnology Co., Ltd), BCA protein quantitation assay kit (Biyuntian Biotechnology Co., Ltd), Primary antibodies: hypoxia inducible factor 1α (HIF-1α) (USA, Affinity Bioscience), SIRT1, Sequestosome 1 (SQSTM1/p62), LC3, Bcl-2, Bcl-2-associated X protein (Bax), caspase-3, β-actin (Aibotech Biotechnology Co., Ltd, Wuhan, China), Secondary antibodies: horseradish peroxidase (HRP) linked anti-rabbit IgG and anti-mouse IgG (Soleibao Biotechnology Company, Beijing, China), Lipofectamin TM 2000 (Invitrogen Company, USA), OPTI MEM medium (Gibco Company, USA), miR-448 mimic/inhibitor or NC (Jima Pharmaceutical Technology Co., Ltd, Shanghai, China), SIRT1 inhibitor EX-527 and Protein Quantitation:Article Title: Apigenin attenuates myocardial ischemia-reperfusion injury through miR-448/SIRT1 axis Article Snippet: .. Cobalt chloride (CoCl 2 ) (Aladdin Biochemical Technology Co., Ltd, Shanghai, China), DMEM high-sugar medium and DMEM sugar-free medium (Gibco Company, USA), FBS (South American, LONSERA company), API (Yuanye Biotechnology Co., Ltd, Shanghai, China), 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) powder (Solaibao Biotechnology Co., Ltd, Beijing, China), caspase-3 enzyme-linked immunosorbent assay (ELISA) kit (ET Life Sciences R&D Co., Ltd), superoxide dismutase (SOD) and lactate dehydrogenase (LDH) measure kit (Jiancheng Bioengineering Research Institute, Nanjing, China), Trizol reagent, ABScript II cDNA Strand Synthesis Kit, Universal SYBR Green Fast qPCR Mix (Aibotech Biotechnology Co., Ltd, Wuhan, China), SIRT1 primers (Dingguo Changsheng Biotechnology Co., Ltd, Beijing, China), miRNA reverse transcription and qPCR kits, miR-448 primers (Hefei, Zhien Biotechnology Co., Ltd), BCA protein quantitation assay kit (Biyuntian Biotechnology Co., Ltd), Primary antibodies: hypoxia inducible factor 1α (HIF-1α) (USA, Affinity Bioscience), SIRT1, Sequestosome 1 (SQSTM1/p62), LC3, Bcl-2, Bcl-2-associated X protein (Bax), caspase-3, β-actin (Aibotech Biotechnology Co., Ltd, Wuhan, China), Secondary antibodies: horseradish peroxidase (HRP) linked anti-rabbit IgG and anti-mouse IgG (Soleibao Biotechnology Company, Beijing, China), Lipofectamin TM 2000 (Invitrogen Company, USA), OPTI MEM medium (Gibco Company, USA), miR-448 mimic/inhibitor or NC (Jima Pharmaceutical Technology Co., Ltd, Shanghai, China), SIRT1 inhibitor EX-527 and |